DNAgard® Blood is designed for the immediate stabilization of DNA in blood with the convenience of room temperature shipping and storage. The preservative reagent rapidly permeates cellular structures and membranes to stabilize and protect genomic DNA in whole blood samples. The use of DNAgard Blood allows a streamlined workflow from blood collection in the clinic or field to sample processing in the laboratory.
Figure-1: DNAgard Blood formulation preserves DNA in whole blood for 14 months. Gel images of isolated genomic DNA recovered from blood samples preserved for 14 months in DNAgard Blood (DGB) at room temperature, in a competitor preservative (COM) at room temperature, unprotected (NP) at room temperature, versus control (-20°C).
DNA protected using the DNAgard Blood formulation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.click the image to expand
Figure 2A . Successful Long-range PCR after 2 months storage. Genomic DNA isolated from human whole blood stored for 60 days in the DNAgard Blood formulation, non-protected (NP) or frozen at -20°C. Long-range PCR amplification of a 22 Kbp amplicon was performed on the DNA (M = 1 kb ladder).
Figure 2B. Real-time PCR after 1 month storage. Genomic DNA isolated from human whole blood stored for 30 days in the DNAgard Blood formulation or frozen at -20°C was quantified using real-time PCR amplification of the b-actin gene.
DNA protected using the DNAgard Blood formation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.click the image to expand
Figure 3. qPCR quantification after 1 month storage of blood, from 3 different donors, in DNAgard Blood formulation (RT), Competitor (RT) or frozen (-20°C).
|Product Formats||10mL, 50mL and 100mL liquid reagents|
|Application volume||1:4 (vol:vol) of stabilizer:blood|
|Blood DNA preservation||At least 14 months at room temperature|
|Product Overview Sheet|
|Five donor study shipping simulation of blood stored in DNAgard® Blood|
|Long-range PCR amplification of DNA from DNAgard® Blood|
|Long-Term Storage of Blood in DNAgard® Blood|
|Sequencing and methylation analysis of blood genomic DNA after extreme temperature exposure in DNAgard® Blood|
|Catalog No.||Product Description||Size|
|62501-026||DNAgard® Blood||10 mL|
|62501-036||DNAgard® Blood||50 mL|
|62501-046||DNAgard® Blood||100 mL|