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VACUETTE® DNAgard® Blood Tube

Collect, Ship and Preserve DNA in Whole Blood at Room Temperature

  • Standard venipuncture collection
  • Preserve and Maintain Sample Integrity for up to 14 months
  • Compatible with different extraction/purification techniques
  • Compatible with all downstream applications
DNAgard Blood Tube

VACUETTE® DNAgard® Blood Tube is designed for the collection, preservation and shipping of whole blood samples for genomic DNA isolation. The preservative reagent rapidly permeates cellular structures and membranes (cell lysis) to stabilize and protect genomic DNA in whole blood samples. The use of VACUETTE DNAgard Blood Tube provides a streamlined workflow from blood collection in the clinic or field to sample processing in the laboratory.

DNAgard Blood Tube - 14 months at room temperature
  • Collect, ship and preserve DNA in blood under optimal conditions
  • Recover high quality DNA (high MW fragments)
  • Recover quantitative DNA (yield)

Product Data


Protect DNA at ambient temperatures



VACUETTE DNA Gard Blood Tube(DGB) Formulation Performance picture click the image to expand

Figure-1: VACUETTE DNAgard Blood Tube formulation preserves DNA in whole blood for 14 months. Gel images of isolated genomic DNA recovered from blood samples preserved for 14 months in DNAgard Blood (DGB) at room temperature, in a competitor preservative (COM) at room temperature, unprotected (NP) at room temperature, versus control (-20°C).


Broad range of applications


DNA protected using the DNAgard Blood Tube formulation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.

dna click the image to expand


Figure 2A . Successful Long-range PCR after 2 months storage. Genomic DNA isolated from human whole blood stored for 60 days in the DNAgard Blood Tube formulation, non-protected (NP) or frozen at -20°C. Long-range PCR amplification of a 22 Kbp amplicon was performed on the DNA (M = 1 kb ladder).

Figure 2B. Real-time PCR after 1 month storage. Genomic DNA isolated from human whole blood stored for 30 days in the DNAgard Blood Tube formulation or frozen at -20°C was quantified using real-time PCR amplification of the b-actin gene.



Representative DNA yield


DNA protected using the DNAgard Blood Tube formation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.

dnaclick the image to expand


Figure 3. qPCR quantification after 1 month storage of blood, from 3 different donors, in DNAgard Blood Tube formulation (RT), Competitor (RT) or frozen (-20°C).

Product Specifications


  • Evacuated, sterile, blood collection tubes (10ml)
  • Pre-fill volume (preservative) = 1.0ml
  • Blood draw volume = 6.5ml
  • Storage temperature = -20°C to 45°C
  • Blood DNA preservation = 14 months at room temperature
  • For Research Use Only. Not for use in diagnostic procedures.
Resources
Product Overview Sheet
Handbook
MSDS
Graphical Instructions for Use (IFU)
Application Notes
Five donor study shipping simulation of blood stored in VACUETTE® DNAgard® Blood Tubes
Long-range PCR amplification of DNA from VACUETTE® DNAgard® Blood Tubes
Long-Term Storage of Blood in DNAgard® Blood
Sequencing and methylation analysis of blood genomic DNA after extreme temperature exposure in VACUETTE® DNAgard® Blood Tubes

Ordering

Catalog No. Product Description Size
53561-038 VACUETTE® DNAgard® Blood Tubes 50-pack
53562-048 VACUETTE® DNAgard® Blood Tubes 100-pack

Buy VACUETTE DNAgard Blood Tubes

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