Forensics

Overview

Biomatrica offers technologies that serve the forensic science community. Through our innovative room temperature storage and preservation of DNA (purified gDNA, blood, buffy coat, tissues and cells) and RNA (tRNA, mRNA, miRNA, siRNA), we provide a cost-effective, scalable and highly validated platform for evidence preservation and protection. In addition, we have developed reagents that enhance PCR and STR performance for difficult/challenging samples (trace levels, degraded, or inhibited).

Evaluations of Biomatrica products by Forensic scientists

Long-term storage of gDNA in DNAstable.

  • Palm Beach County Sheriff's Office - Dr. Cecilia A. Crouse, Chief Scientific Officer
    • The study involved a rigorous evaluation of stabilization of forensic DNA samples using Biomatrica's DNAstable (room temperature) and cold freezers (-20C), at various sample concentrations (625 pg to 1 ng), and at different time points - 5 days, 7 days, 2 weeks, 1 month, 2 months, 3 months, and 1 year. A highlight of the results obtained in the study is shown below:
    Graph shows the % recovery of DNA stored at room temperature on Biomatrica Plates (SM) compared to freezing at -20C over time for mixture data.

    • The study was presented at the workshop on "Advances in Forensic DNA Analysis", 62nd AAFS Annual Scientific Meeting, Seattle, WA, Feb 22, 2010.
    • You can download the full presentation here.
  • San Diego County Sheriff's Department - Steve Guroff, Supervising Criminalist
    • 2 DNA samples extracted from blood were preserved in DNAstable at room temperature, and frozen at -20C (control) for 3.5 years. After rehydration (DNAstable-matrix) and freeze/thaw (cold-frozen), the samples were analyzed by PCR and the results are displayed below.

Key Customers

  • Washington State Patrol, WA.
  • Palm Beach County Sheriff's Office, FL.
  • Fresno California Sheriff's Office, CA.

List of publications, scientific posters, and application notes

  • Lee, SB, Clabaugh, KC, Silva, B, Odigie, KO, Fourney, RM, Stevens, J, Carmody, GR, Coble, MD, Loreille, O, Scheible, M, Parsons, TJ, Pozder, A, Eisenberg, AJ, Budowle,B, Taha Ahmad, Russell W. Miller, Amy B. McGuckian, Julie Conover-Sikorsky and Cecelia A. Crouse. Assessing a novel room temperature DNA storage medium for forensic biological samples. 2010. Forensic Science International - Genetics. (Submitted 12/09 and provisionally accepted with revisions pending).
  • Lee SB, Crouse, CA and Kline MC: Optimizing Storage and Handling of DNA extracts; Forensic Sci Rev, 2010 (Submitted March 2010 and accepted with minor revisions).
  • Thomas Doedt, QIAsafeTM DNA - An innovative Anhydrobiosis-based technology for long-term storage of DNA Proceedings of the workshop on Long-term storage of DNA Material, August 2009.
  • Bruce Budowle and Angela van Daal, Extracting evidence from forensic DNA analyses: future molecular biology directions, Biotechniques, 2009, 46 (5), 339-350.
  • Peggy Ho Faix and Althea Lee, How to improve recovery of DNA profiles, Evidence Technology Magazine, Vol 7 (#2), March-April 2009, pg. 28-31.
  • Steven B. Lee and Kathy Roberts, Saving your samples: Room temperature stabilization of DNA and RNA, Emerging technologies in Forensic Science, 2008, Vol 1, pg. 8-10.
  • Linda Le, Kimberly Clabaugh, Clarisa Trogdon, Andrew Chang, Marissa Meininger, Michael D. Coble, Odile Loreille, Melissa Scheibel, Rachel Demara, Sohela de Rozieres, Martin Latterich, Rolf Muller and Steven B. Lee. Recovering DNA profiles from low quantity and low quality forensic samples Recovering DNA profiles from low quantity and low quality forensic samples. A poster presentation at the 19th International Symposium on Human Identification meeting, Holywood, CA, October 2008.
  • Kimberly Clabaugh, Brie Silva, Kingsley Odigie, Ron Fourney, Jesse Stevens, George Carmody, Mike Coble, Odile Loreille, Melissa Scheible, Margaret Kline, Thomas Parsons, Arijana Pozder, Arthur Eisenberg, Bruce Budowle and Steven B. Lee. 2007 and 2008 Storage of DNA samples at ambient temperature using DNA-SampleMatrix. Poster presented at the 18th Annual International Symposium on Human Identification. October, 2007, Hollywood, CA. Also presented as an oral presentation at the Fall 2007 California Association of Criminalists Meeting held in Berkeley, CA October 2007, the American Academy of Forensic Sciences February 2008 meeting held in Washington DC. Published in the Proceedings of the American Academy of Forensic Sciences. Volume 14. page 51 - and the Spring 2008 California Association of Criminalists meeting, May 2008, San Diego, CA.
  • R. Muller, J. Muller-Cohn and SB Lee. 2007 Optimizing DNA Stabilization and Storage of Forensic DNA Samples using polymers. Poster presented at the 18th Annual International Symposium on Human Identification. Held October, 2007. Hollywood, CA.

Contact

For more information about Biomatrica products please contact us at contact@biomatrica.com or call 1-858-550-0308.

 

abdul rahman el assir