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RNAstable Protocol
RNAstable™ preserves RNA samples at room temperature. Each tube or plate contains RNAstable as a coating at the bottom of the tube or well, which protects picogram to microgram amounts of RNA. This medium is completely dissolvable and ensures total sample recovery.
Stabilize for Storage.
- Open cap on the tube or remove seal from 96-well plate.
- Add up to 100 µl [≤100 µg] of your RNA sample directly to each tube or well.
- Leave tube open to dry overnight in laminar flow hood.
- For volumes ≥20 µl, or for accelerated drying, please SpeedVac®, without heat.
| Sample Volume | Drying Time |
| 10-20 µl | 30 minutes |
| ≥20µl-30 µl | 1 hour |
| ≥30µl-100 µl | 1.5 hours |
- Once dried, cap tube or seal 96-well plate, place in the moisture-barrier
bag, and
reseal for storage on your benchtop for up to 1 month at room temperature (15-
25°C) with ≤50% relative humidity. - For long-term storage >1 month, you may
either store the dried samples in a
desiccating chamber, or heat seal the moisture-barrier bag containing the dried samples
and a desiccant pack. Store at room temperature (15-25°C) with ≤50% relative humidity.
(Additional sets of 12 sample pouches with desiccant packs are available from
www.biomatrica.com, catalog # 14001-087.)
Sample Recovery: Just Add Water.
- Add 10-100 µl of H2O or other liquid to the tube or well containing stored sample.
- Hydrate for 15 minutes.
- Pipette gently to ensure complete mixing
| Samples can be used directly in downstream applications. |
|
To download a printable version of this protocol, click here.