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DNA Stable Plus A DNA Preservation Product

RNAstable®

Stabilize and store RNA at Ambient Temperatures

  • Preserves RNA at ambient temperatures without degradation
  • Eliminates the need to store RNA in freezers or to ship on dry ice
  • Concentrates RNA more effectively than other methods
  • Compatible with most downstream applications



RNAstable

RNAstable® and RNAstable® LD allows you to preserve and ship your total RNA, mRNA, and miRNA for up to 12 years at ambient temperatures*. They provide a cost-effective, eco-friendly solution to freezer crawl and lab space management. Moreover, they elimnate sample loss due to freeze/thaw cycles. When you are ready to use your RNA samples, you can fully recover them in minutes without any additional purification steps.

RNAstable is available in two convenient formats:

  • RNAstable: provided pre-dried in 1.5 ml microfuge tubes and 96-well plates to which hydrated RNA can be added.
  • RNAstable LD: a liquid reagent which can be added directly to RNA samples in your own tubes, multi-well plates, or other containers.
RNAstable and RNAstable LD Workflow

*Based on accelerated aging studies

Product Performance


Figure 1: RNAstable protects total RNA, mRNA, miRNA. Samples can be safely stored for 12 years at ambient temperature and protected from degradation.
RNAstable Agilent 2100 and gel data
Figure 2: Successful gene expression analysis on microarray.
Gene expression analysis on microarray
Figure 3: (A) Aliquots of total RNA were stored for 29months at either -80°C or at room temperature protected in RNAstable or unprotected. RNA integrity was assessed using agarose gel electrophoresis and bioanalyzer analysis (e.g. RIN score). (B) Aliquots of the stored samples were quantified using the 18S rRNA gene in a One-Step RT-PCR reaction. Three samples were amplified for each storage condition and the results from the 9 reactions are overlaid on the graph. (C) Recovery levels (pg) for total RNA stored under identical conditions.

Product Specifications


Product formats
  • Pre-dried in 1.5 ml microfuge tubes and 96-well plates (RNAstable)
  • Liquid aliquots of 2 ml and 10 ml (RNAstable LD)
Application volume 100µl
Application amount 0.01 to 100µg
Drying time Overnight (laminar flow hood); 0.5 to 1.5 hours with vacuum concentrator system (speedVac®)
Recovery volume 10 to 100µl
Recovery ~99.9%
DNA storage in liquid at RT 12 years accelerated aging; 2.5 years "real time"
Resources
 
The following documents are available for download:
RNAstable® Overview
RNAstable® Quick Reference Protocol
RNAstable® / RNAstable® LD Handbook
RNAstable® MSDS
RNAstable® LD MSDS
RNAstable® LD Quick Reference Protocol
RNAstable® Frequently Asked Questions (FAQ's)
Application notes for download:
RNA Stabilization at Room Temperature in RNAstable®
Improved QPCR Analysis Following Storage in RNAstable®
Microarray Analysis of RNA in RNAstable®
microRNA (miRNA) Stabilization in RNAstable®
Stabilization of poly(A) mRNA transcripts in RNAstable®
Articles available for download:
Summary of research evaluations of RNA preservation in RNAstable® versus cold storage (at -80°C)
BioTechniques - June 2010 - Stabilizing RNA at room temperature in RNAstable®
BioTechniques - August 2009 - Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis

Ordering

Catalog No. Product Description Size
93220-001 RNAstable® Trial Kit (3) Tubes of RNAstable®
93221-001 RNAstable® Tube Kit (25) Tubes of RNAstable®
93221-011 RNAstable® Tube Kit 3 x (25) Tubes of RNAstable®
90220-001 RNAstable® 96-Well Plate (1) 96-well plates
90222-001 RNAstable® 96-Well Plate Pack (10) 96-well plates
53201-013 RNAstable® LD 2ml (1) 2ml bottle of liquid RNAstable® for dry storage
52201-013 RNAstable® LD 10ml (1) 10ml bottle of liquid RNAstable® for dry storage
52201-033 RNAstable® LD (5 X 10ml) (5) 10ml bottle of liquid RNAstable® for dry storage

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