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Description
Genomic DNA Data
Plasmid DNA Data
Downstream Applications
Application Notes
Protocol

 

DNA SampleMatrix® Protocol - Now Available as QIAsafe™

Protocol

Objective: Preserve plasmid and genomic DNA.

Storage:

  1. Open tube or remove seal from plate
  2. Add 1-20μl of DNA to tube or well (from picograms up to 30μg)
  3. Dry samples completely at room temperature

    • Overnight on the bench-top or in laminar flow hood
    • Faster drying times can be achieved using a vacuum (refer to Biomatrica drying guidelines)

  4. Store dried samples in an airtight container at room temperature.

    •For optimal results dry samples should be sealed with an ambient humidity of less than
      50%.

Sample Recovery:

  1. Add 10-50 μl of water tube or well
  2. Hydrate for 15 minutes, mixing not necessary
  3. Use directly in downstream application
  4. Sample can be re-dried to preserve remaining DNA. Refer to storage steps 2&3.

 

Printable Protocol

 

Drying samples in SampleMatrix® with a Speedvac

Protocol

Objective: Preserve plasmid and genomic DNA samples in SampleMatrix® by drying
samples down using a Speedvac.

Appropriate drying times:

Volumes to
SpeedVac
Recommended Drying Time
10-20μl 30 minutes
>20μl-30μl 1 hour
>30μl-50μl 1 ½ hours
>50μl-100μl 1 ½ hours

*Please note that no heat is used while drying down your DNA samples.

Printable Protocol

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