DNA SampleMatrix® Protocol - Now Available as QIAsafe™
Protocol
Objective: Preserve plasmid and genomic DNA.
Storage:
- Open tube or remove seal from plate
- Add 1-20μl of DNA to tube or well (from picograms up to 30μg)
- Dry samples completely at room temperature
• Overnight on the bench-top or in laminar flow hood
• Faster drying times can be achieved using a vacuum (refer to
Biomatrica drying guidelines)
- Store dried samples in an airtight container at room temperature.
•For optimal results dry samples should be sealed with an ambient
humidity of less than
50%.
Sample Recovery:
- Add 10-50 μl of water tube or well
- Hydrate for 15 minutes, mixing not necessary
- Use directly in downstream application
- Sample can be re-dried to preserve remaining DNA. Refer to storage
steps 2&3.
Printable Protocol
Drying samples in SampleMatrix® with a Speedvac
Protocol
Objective: Preserve plasmid and genomic DNA samples in SampleMatrix® by drying
samples down using a Speedvac.
Appropriate drying times:
Volumes to
SpeedVac |
Recommended Drying Time
|
| 10-20μl |
30 minutes |
| >20μl-30μl |
1 hour |
| >30μl-50μl |
1 ½ hours |
| >50μl-100μl |
1 ½ hours |
*Please note that no heat is used while drying down your DNA samples.
Printable Protocol
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