LBgard Blood Tubes are CE marked. [...]
Yes, gDNA can be extracted from blood collected in LBgard tubes using the QIAamp blood isolation kit (51106). gDNA yield can be improved if necessary [...]
Protocols: Do we need to perform an additional fixation step with PFA prior to storing or staining by immunofluorescence?
For slide immunofluorescence assays and flow cytometry assays, we recommend a fixation step. [...]
Standard red blood cell lysis protocols. [...]
Protocols: What kit is recommended for cell free DNA isolation for plasma isolated from LBgard tubes?
We recommend the QIAamp CNA kit (55114) from Qiagen. Other kits validated for use with LBgard tubes are the Norgen Plasma Circulating DNA Purification [...]
Yes, centrifuge at 3000 rcf for 15 minutes. [...]
Invert LBgard tubes containing whole blood to mix. Spin at 1900 rcf for 10 minutes. Transfer top plasma layer carefully to an empty conical tube, care [...]
No, freezing blood in LBgard tubes causes cell lysis. [...]
Sample Collection: How long we should wait after the blood draw to consider the sample fully stabilized/preserved?
After inverting the tube 10X (see IFU), blood is fully stabilized. [...]