Protocols: What is the recommended protocol for isolating plasma from LBgard tubes?

By |May 30th, 2018|0 Comments
  1. Invert LBgard tubes containing whole blood to mix.
  2. Spin at 1900 rcf for 10 minutes. Transfer top plasma layer carefully to an empty conical tube, carefully avoiding the buffy coat and red blood cell layers.
  3. Spin extracted plasma at 4500 rcf for 15 minutes. Transfer the plasma to an empty tube avoiding any remaining pellets.
  4. Repeat centrifugation Step 3, if desired.

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