Brake settings are centrifuge-dependent. For the first spin, we recommend light braking, which will not disturb the buffy coat. For the second spin, h [...]
Yes, gDNA can be extracted from blood collected in LBgard tubes using the QIAamp blood isolation kit (51106). gDNA yield can be improved if necessary [...]
Do we need to perform an additional fixation step with PFA prior to storing or staining by immunofluorescence?
For slide immunofluorescence assays and flow cytometry assays, we recommend a fixation step. [...]
Standard red blood cell lysis protocols. [...]
We recommend the QIAamp CNA kit (55114) from Qiagen. Other kits validated for use with LBgard tubes are the Norgen Plasma Circulating DNA Purification [...]
Yes, refer to application note Confirmation of Equivalence of One-Spin and Two-Spin Protocols for Plasma Isolation from LBgard Blood Tubes [...]
Invert LBgard tubes containing whole blood to mix. Spin at 1900 rcf for 10 minutes. Transfer top plasma layer carefully to an empty conical tube, care [...]