Yes, gDNA can be extracted from blood collected in LBgard tubes using the QIAamp blood isolation kit (51106). gDNA yield can be improved if necessary [...]
Do we need to perform an additional fixation step with PFA prior to storing or staining by immunofluorescence?
For slide immunofluorescence assays and flow cytometry assays, we recommend a fixation step. [...]
Standard red blood cell lysis protocols. [...]
We recommend the QIAamp CNA kit (55114) from Qiagen. Other kits validated for use with LBgard tubes are the Norgen Plasma Circulating DNA Purification [...]
Yes, centrifuge at 3000 rcf for 15 minutes. [...]
Invert LBgard tubes containing whole blood to mix. Spin at 1900 rcf for 10 minutes. Transfer top plasma layer carefully to an empty conical tube, care [...]