Frequently Asked Questions (FAQs)
Brake settings are centrifuge-dependent. For the first spin, we recommend light braking, which will not disturb the buffy coat. For the second spin, heavy braking is ok.
Yellowing of color and decrease in additive volume are expected over the lifetime of the LBgard Blood Tube. These changes do not affect functional performance of the tube. Performance is validated for any tube used before its expiration date, provided it has been stored within the indicated temperature range.
LBgard Blood Tubes are CE marked.
Yes, gDNA can be extracted from blood collected in LBgard tubes using the QIAamp blood isolation kit (51106). gDNA yield can be improved if necessary by increasing Proteinase K treatment time.
For slide immunofluorescence assays and flow cytometry assays, we recommend a fixation step.
Standard red blood cell lysis protocols.
We recommend the QIAamp CNA kit (55114) from Qiagen. Other kits validated for use with LBgard tubes are the Norgen Plasma Circulating DNA Purification kit (55100), the QIAamp MinElute kit (36912), the MagMax kit (A29319), the Epi proColon plasma quick kit (M5-02-001), and the Roche cobas cfDNA sample preparation kit (07247737190).
Yes, refer to application note Confirmation of Equivalence of One-Spin and Two-Spin Protocols for Plasma Isolation from LBgard® Blood Tubes
- Invert LBgard tubes containing whole blood to mix.
- Spin at 1900 rcf for 10 minutes. Transfer top plasma layer carefully to an empty conical tube, carefully avoiding the buffy coat and red blood cell layers.
- Spin extracted plasma at 4500 rcf for 15 minutes. Transfer the plasma to an empty tube avoiding any remaining pellets.
- Repeat centrifugation Step 3, if desired.
No, freezing blood in LBgard tubes causes cell lysis.
After inverting the tube 10X (see IFU), blood is fully stabilized.
Yes- it works for short draws down to 4 mL.
4°C-25°C in a sealed pack.
Shelf life is validated to 14 months. Slight yellowing of formulation or decreases in volume during this time do not impact product performance.
Approximately ~0.56 mL of stabilizer solution.
Yes, they meet an SAL of 10-6.
Up to 14 days for cell-free DNA applications when stored between 4°C – 37°C. Up to 4 days for nucleated cell applications (CTC, WBC and fetal cells) when stored between 18°C and 30°C.
LBgard Blood Tubes are direct-draw blood collection tubes pre-filled with a proprietary reagent that stabilizes cells and cell-free DNA (cfDNA) in whole blood, preserving biological analytes for downstream applications. LBgard Blood Tubes also prevent the release of cellular components that can interfere with analysis, such as cellular genomic DNA and products from hemolysis. LBgard enables shipment of whole blood samples at ambient temperature from the phlebotomy site to the testing lab.
Yes. PCRboost™ enhances PCR reactions with as little as 10 pg of genomic DNA or 50-100 ng of RNA.
We are currently working on formulations for various downstream applications, including other polymerases. Please refer to our website (https://www.biomatrica.com/) for the latest updates on current and future product information.
PCRboost™ can be used for multiplex PCR and we are currently analyzing its ability to amplify GC-rich templates. Please refer to our website (https://www.biomatrica.com/) for the latest updates.
PCRboost™ does not change the fidelity of polymerases nor does it have an effect on the accuracy of the amplicon sequence. For high fidelity amplification, we recommend the use of a proofreading polymerase.
PCRboost™ has been shown to amplify DNA in presence of PCR inhibitory factors, resulting in 30-50% better amplification.
In case a PCR reaction product containing PCRboost™ needs to be read by UV spectroscopy, it is highly recommended to blank the spectrophotometer against a PCRboost™ sample prior to taking the OD measurements.
You will notice a white pellet (DNA and PCRboost™ components) after ethanol precipitation. Resuspending the pellet in buffer or water redissolves the pellet and does not interfere with performance of the DNA.
No. PCRboost™ does not interfere or inhibit downstream applications, so there is no need to purify the amplified product.
PCRboost™ works with the majority of commercially available Taq polymerases.
It is recommended that PCRboost™ be added to the second-strand RT-PCR reaction. In some cases, however, it may also be advantageous to supplement the first-strand reaction with PCRboost™ instead of water, particularly when attempting to amplify rare transcripts.
PCRboost has a shelf life of 6 months.
No. Simply add PCRboost™ to your PCR cocktail to bring up to volume and run your standard protocol.
Simply substitute PCRboost™ for water to bring your reaction up to volume. For example, if you normally add 20 µl of water per reaction, just add 20 µl of PCRboost™ instead.
Any oligos will work with PCRboost™. Follow your standard PCR reaction protocol and amplification conditions.
PCRboost™ should be stored at room temperature for best performance. Once frozen, a precipitate may form in the liquid, but the function of the PCRboost™ is not affected. Mix thoroughly before use and store at room temperature.
PCRboost™ should be stored at room temperatures between 15°C to 25°C.
As a “drop in” reagent, PCRstable® can be incorporated into any manufacturing workflow. Biomatrica works closely with each customer to help implement PCRstable® in their manufacturing processes.
The service timeline varies based on the assay and customer requirements. Typically, the process requires a minimum of 90 days.
Please see the PCRstable® Concept to Product Overview, which outlines the process for a PCRstable service, or call Biomatrica and we would be happy to walk you through it.
Lyophilization is the process of creating a stable preparation of a substance by rapid freezing and dehydration of the frozen product under high vacuum. PCRstable® overcomes the manufacturing challenges of producing cartridges using lyophilized reagents. Unlike lyophilization, PCRstable® reagents are prepared at ambient temperatures and stable in higher humidity. They are amenable to any chip, cartridge, or cassette platform, and don’t require difficult handling with tweezers like lyospheres.
PCRstable® chemistry can stabilize any type of PCR-based assay, including, molecular diagnostic, point-of-care and lab-on-a-chip assays with unique formats.
No. PCRstable® is a custom service that screens a library of proprietary chemical stabilizers to identify the optimal formulation for long-term, temperatureindependent, stability of wet or dry PCR reagents in your unique assay.
PCRstable® is service that will develop custom chemical stabilizers as an alternative to lyophilization for PCR-based assay stabilization. Manufacturers add PCRstable® chemical stabilizers to any PCR reagent, from polymerases up to complete master mixes, and the PCR reagents remain stable for years at room temperature. It’s ideal for developing point-of-care, molecular diagnostic platforms or next gen cartridge/chip-based instruments.
The PCRstable® service screens a library of chemical stabilizers to identify the optimal formulation for long-term stability of your wet or dry PCR reagents at any temperature. You simply add the formulation to your reagent mix in any vessel ─ plastic, chip, cartridge ─ air dry if specified, and store or ship at any temperature.
The reaction is not affected. RNAstable® contains a pH indicator that turns pink when added to a slightly basic reaction mix.
Animal, plant, and viral RNA.
Place dried RNAstable® samples in a foil sealed bag with desiccant. Place foil bag in an envelope and ship. No ice is required.
Samples are to be stored in a dry storage cabinet or a foil sealed bag with desiccant.
These are included with the kit if RNase contamination is a concern when drying RNA samples in a Vacufuge. You can view a video of this procedure here: http://youtu.be/a7uDSxW1Ap4
No. RNA will only stabilize high quality RNA samples with a RIN of 8.0 to 10.0.
RNAstable® fluoresces in the 230nm to 260nm range. Simply use an empty tube of RNAstable® as a blank for UV-Spectrophotometry and Nanodrop readings.
RNAstable® is designed for shipping and long term storage of RNA. RNAconcentrator is designed for concentrating RNA samples and used for shipping and short term storage up to 1 week.
We recommend up to 3 times of drying and rehydration.
No, extended drying will not cause any damage.
Touch the yellow matrix with an RNAase-free pipette tip. If yellow does not stick to the tip, then RNAstable is completely dry. If yellow sticks to the tip, then RNAstable® is not completely dry. Allow sample to continue drying.
100pg to 100ug of RNA per tube or well.
SalivaGard HT DNA
SalivaGard HT DNA is a saliva DNA collection device optimized for laboratory processing with automated instruments. Its pierce-able cap and triple redundant labeling make it ideal for laboratories performing high-throughput DNA analysis. Additionally, SalivaGard HT DNA features a unique, user-friendly design, with a cap-integrated stabilizer and leak prevention features to improve donor compliance.
The triple redundant labeling, which is CDC compliant and LIMS compatible includes a laser etched barcode on the side of the tube, a 2D barcode on the bottom of the tube, and a readable label on the side of the tube.
The device features integrated stabilizer, which is released from the funnel lid into the saliva sample in the tube when donors screw on the lid. Additionally, the device features a wide funnel for cleaner saliva collection, integrated stabilizer to avoid user contact, a tethered cap to prevent loss, and a wide base to prevent spills.
SalivaGard HT DNA tubes are CE marked.
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