AssayStable® & PCRstable®

Precise positioning and controlled release of assay reagents not achievable by lyophilization

AssayStable and PCRstable are ambient temperature chemistries that allows assay manufacturers to precisely position, preserve, and release their assay reagents in any format using Biomatrica’s unique reagents with a simple air-dry process. PCRstable was developed for PCR-based assays, and AssayStable was developed for all other assays.

Overview

Assay Manufacturers:

  • Air-dry any assay reagent in-house and store at room temperature
  • No lyophilization and no low humidity chambers needed
  • Virtually any reagent stabilized by patented stabilizers

Assay Users:

  • Prepare any assay in a dry-stable format
  • Add template and go; no mixing needed
  • Rapid rehydration upon template addition

Use Cases

  • GenMark Diagnostics: Valve-Free Positioning and Preserving of Sample-to-Answer Diagnostic Devices
  • Thermo Fisher Ion AmpliSeq™: Preservation of High Throughput Next Generation Sequencing Arrays
  • Focus Diagnostics: Enabling decentralized, point-of-care diagnostics through chemical biostabilization
  • Extending shelf-life of one-step, liquid RT-PCR assays for point of care
  • Instantaneous Preservation of Rare Cells in Blood to Evaluate Cancer Therapy Selection
  • PCR Assay Mastermix Ambient Stabilization Without Lyophilization
  • Stabilization of Multiplexed Panel for Next-Generation Sequencing

Product Data

Stabilization of qPCR assay

Included in dry-down:

  • Taq
  • Primers
  • Reverse Transcriptase
  • Dye (SYBR® Green)/Taqman®
  • dNTPs
  • Salts and Detergent

Stabilization of qPCR assay at ambient temperature

Figure 1: Components of qPCR assay, including Taq DNA polymerase, dNTPs, primers for RNase P, SYBR Green dye and PCR buffer were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C. At determined time points, samples were rehydrated with water and MgCl2 with the specified amount of genomic DNA template. Real time PCR amplification of a RNase P fragment was performed on the ABI 7300 Real-Time PCR System, using the equivalent amount of DNA with qPCR reagents stored at -20°C as a positive control. Data shown is for samples stored for 6 months at 45°C, corresponding to 2 years of ambient temperature storage, based on accelerated aging.

Stabilization of RT-qPCR assay at ambient temperature

Figure 2: All components of a RT-qPCR assay, including Taq DNA polymerase with reverse transcriptase activity, dNTPs, aptamer and PCR buffer including metal co-factor were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C. At determined time points, samples were rehydrated with water, primers and TaqMan probes, and a known input of RNA template was added. Reverse transcription and real time PCR amplification were performed on ABI 7300 Real-Time PCR System, using the equivalent amount of RNA samples with RT-qPCR reagents stored at -20°C as a positive control. Data shown is for samples stored for 3 weeks at 45°C, corresponding to 3 months of ambient temperature storage, based on accelerated aging.

qPCR Stabilized

Figure 3: Taq + dNTPs stabilized for 4 years at room temperature(based on accelerated aging).

Stabilization of a Limiting Quantity of Reverse Transcriptase

Figure 4: Stabilization of a limiting amount of reverse transcriptase for rapid screening ability.

Ambient Shipping: Stress Testing

Figure 5: Biomatrica stabilizers protected all samples, while non-protected shows complete degradation below 10 ng.

Specifications

Resources

PCRstable

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AN - Ambient temperature stabilization of an HBV real-time PCR assay using PCRstable technology 153.05 KB

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AN - HawkZ05-based HIV/HCV real-time RT-PCR assays stabilization with PCRstable® technology 152.62 KB

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AN - Preparation of dry, stable RT-PCR assay reagents with PCRstable® technology using a vacuum concentrator 411.84 KB

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AN - Stabilization of RT-qPCR Reagents at Ambient Temperatures 153.28 KB

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Brochure - PCRstable® 923.87 KB

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Influenza real-time RT-PCR assays stabilized with PCRstable technology 172.51 KB

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PCRstable Overview Sheet 923.87 KB

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PCRstable vs. Lyophilization Comparison 819.30 KB

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Performance of a PCRstable-stabilized real-time PCR C. diff DNA assay 185.69 KB

    AssayStable

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    AN - Ambient temperature stabilization of an HBV real-time PCR assay using PCRstable technology 153.05 KB

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    AN - HawkZ05-based HIV/HCV real-time RT-PCR assays stabilization with PCRstable® technology 152.62 KB

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    AN - Preparation of dry, stable RT-PCR assay reagents with PCRstable® technology using a vacuum concentrator 411.84 KB

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    AN - Stabilization of RT-qPCR Reagents at Ambient Temperatures 153.28 KB

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    AssayStable Overview Sheet 1,007.74 KB

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    Brochure - AssayStable™ 1,007.74 KB

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    Influenza real-time RT-PCR assays stabilized with PCRstable technology 172.51 KB

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    Performance of a PCRstable-stabilized real-time PCR C. diff DNA assay 185.69 KB

      Ordering

      For information on these products, please inquire.

      BIOMATRICA
      5627 Oberlin Drive
      Suite 120
      San Diego, CA 92121
      Tel: 858-550-0308
      Fax: 858-678-0597
      Email: orders@biomatrica.com