DNAgard® Blood Tubes

More cost effective than EDTA

  • Optimized for DNA methylation, PCR and NGS assays
  • Preserve and Maintain Sample Integrity for 3 months
  • Compatible with multiple extraction/purification techniques
  • Compatible with all downstream applications

Overview

DNAgard® Blood Tube is designed for the collection, preservation and shipping of whole blood samples for genomic DNA isolation. The preservative reagent rapidly permeates cellular structures and membranes (cell lysis) to stabilize and protect genomic DNA in whole blood samples. The use of DNAgard Blood Tube provides a streamlined workflow from blood collection in the clinic or field to sample processing in the laboratory.

Collect, ship and preserve DNA in blood under optimal conditions
Recover high quality DNA (high MW fragments)
Recover quantitative DNA (yield)

Product Data

Protect DNA at ambient temperatures

Figure-1: DNAgard Blood Tube formulation preserves DNA in whole blood for 3 months. Gel images of isolated genomic DNA recovered from blood samples preserved for 14 months in DNAgard Blood (DGB) at room temperature, in a competitor preservative (COM) at room temperature, unprotected (NP) at room temperature, versus control (-20°C).

Broad range of applications

DNA protected using the DNAgard Blood Tube formulation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.

Figure 2A . Successful Long-range PCR after 2 months storage. Genomic DNA isolated from human whole blood stored for 60 days in the DNAgard Blood Tube formulation, non-protected (NP) or frozen at -20°C. Long-range PCR amplification of a 22 Kbp amplicon was performed on the DNA (M = 1 kb ladder). Figure 2B. Real-time PCR after 1 month storage. Genomic DNA isolated from human whole blood stored for 30 days in the DNAgard Blood Tube formulation or frozen at -20°C was quantified using real-time PCR amplification of the b-actin gene.

Representative DNA yield

DNA protected using the DNAgard Blood Tube formation is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.

Figure 3. qPCR quantification after 1 month storage of blood, from 3 different donors, in DNAgard Blood Tube formulation (RT), Competitor (RT) or frozen (-20°C).

Specifications

Evacuated, sterile, blood collection tubes (10ml)
Pre-fill volume (preservative) = 1.0ml
Blood draw volume = 6.5ml
Storage temperature = -20°C to 45°C
Blood DNA preservation = 3 months at room temperature
For Research Use Only. Not for use in diagnostic procedures.

Resources

DNAgard Blood Tube

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AN - Five donor study shipping simulation of blood stored in DNAgard® Blood Tubes 232.24 KB

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AN - Long-range PCR amplification of DNA from DNAgard® Blood Tubes 141.00 KB

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AN - Long-Term Storage of Blood in DNAgard® Blood 117.33 KB

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AN - Sequencing and methylation analysis of blood genomic DNA after extreme temperature exposure in DNAgard® Blood Tubes 202.62 KB

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DNAgard Blood Tube - Instructions 281.31 KB

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DNAgard Blood Tube - Material Safety Data Sheet 69.70 KB

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DNAgard Blood Tube - Product Overview Sheet 714.29 KB

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DNAgard Blood Tube User Manual 846.25 KB

    Ordering

    • Catalog No.: 53561-038
    • Description: DNAgard® Blood Tubes
    • Size: 50-pack
    • Price: $45.00
    • Order Here

    BIOMATRICA
    5627 Oberlin Drive
    Suite 120
    San Diego, CA 92121
    Tel: 858-550-0308
    Fax: 858-678-0597
    Email: orders@biomatrica.com