DNAstable® Plus enables you to preserve, store and ship your purified DNA samples at room temperature in liquid and liquid-to-dry formats. It offers flexibility with thermostability at extreme temperatures, and the ability to handle high concentrations of DNA. Recovery from the dry matrix is achieved within minutes by simply adding water. Your DNA sample is ready for downstream analysis without the need for any additional purification steps.
DNA stabilization can be achieved in two formats:
Liquid Stabilization (short-term)
Liquid-to-Dry (LD) Stabilization (long-term)
Stability in Liquid Format:
Figure 1: The gel image above shows protection of DNA samples stored in DNAstable Plus (conc: 50 ng/ul) for 6 months at 45°C in liquid format compared to unprotected samples (TE; no visible bands). The positive controls are samples stored at 4°C.
Stability in Liquid-to-Dry (LD) Format:
Figure 2: Stability for 3 years at 60°C Samples 1-3 and 10-12 above represent DNA stored in TE buffer for 3 years at 4°C in liquid format (positive control). The remaining samples were stored dry at 60°C for 3 years. Samples 4-9 represent 50 ng/µL DNA stored in either DNAstable Plus (4-6) or TE (7-9). Samples 13-18 represent 5 ng/µL DNA stored in either DNAstable Plus (13-15) or TE (16-18). 100 ng of the triplicate samples per condition were run on a 1% agarose gel stained with ethidium bromide.
Figure 3: Stability for 6 months at 85°C The gel image above shows protection of DNA samples stored in DNAstable Plus (conc: 50 ng/ul) for 6 months at 85°C in liquid-to-dry format compared to unprotected samples (NP; no visible bands). The positive controls are samples stored at 4°C.
Figure 4: Long-range PCR assays using DNA samples stored for 3 years in DNAstable Plus Samples 1-3 above represent DNA stored for 3 years in TE buffer at 4°C in liquid format while the rest of the samples were stored dry at 60°C for 3 years at 50 ng/µL. Samples 4-6 represent DNA stored in DNAstable® Plus, while samples 7-9 represent DNA stored in TE buffer. 100 ng of rehydrated DNA from triplicate samples were amplified using KOD Xtreme™ Hot Start PCR kit (Novagen) with 7.5 kb human β-globin primers.
Downstream Analysis (qPCR) of genomic DNA stored dry for 6 months at various “STRESS” conditions.
DNAstable Plus protects genomic DNA under extreme stress conditions at +85°C in LD format.
DNAstable Plus does not cause any interference with downstream analysis.
DNA recovered from samples protected with DNAstable Plus (stressed at 60°C or 85°C) are similar to DNA recovered from stored at 4°C.
Figure 5: Human genomic DNA samples were stored for 6 months in a number of conditions, recovered and compared. From left to right: Control 4°C; Stored in DNAstable Plus at 60°C; Stored in DNAstable Plus at 85°C; Stored unprotected (TE) at 60°C; and Stored unprotected (TE) at 85°C (no DNA recovered). The control was a liquid whereas all other samples were recovered from a dried state.
Figure 6: qPCR traces of DNA recovery experiments, described above in Figure 5. Representative traces from triplicate samples are shown.
Figure 7: DNA recovered after storage at +85°C for 3 months in dried format. Samples protected with DNAstable Plus (DSP) are recovered similar to -20°C controls. However, non-protected (NP) samples and competition “G” show no DNA recovery.
Product Formats: 2ml and 10ml Liquid Reagents
Application volume: 1:4 (vol:vol) of stabilizer:liquid DNA
Application amount: 0.001 to 250µg
Drying time: 10 min (SpeedVac) to overnight (Laminar hood
Recovery volume: 10 to 200µl
DNA storage in liquid at RT: 1 year (accelerated studies, 6 months for plasmid DNA)
DNA storage in dried format at RT: At least 40 years (accelerated studies)