RNAgard® Blood Tubes

RNA and DNA for Gene Expression From One Sample

  • Superior yield and quality of RNA
  • Enhanced gene expression stability
  • Maintain RNA for 14 days at 18 – 25°C or 7 weeks at 4 – 8 °C
  • Compatible with multiple downstream applications
  • Optimized RNAgard Blood Purification Kit available

 

Overview

Preserve RNA Instantly

The RNAgard Blood System is designed for the collection, preservation and purification of RNA and DNA from whole blood samples. The RNAgard Blood preservative reagent in the tubes rapidly permeates and lyses cellular structures and membranes to stabilize and protect RNA and DNA from degradation. Preservation is effective for 14 days at room temperature and 1 month at 4°C.

RNAgard Blood Tubes, CE are For Export Only. Not for sale in the U.S. Not for DNA isolation.

Product Data

Protect RNA instantly at ambient temperatures

Figure 1. Gene expression analysis of blood RNA preservation after 3 and 14 days in RNAgard Blood (RGB). Human blood from 2 healthy donors was collected in RNAgard tubes or competitor’s RNA preservation tubes. RNA was isolated on collection day and after 3 or 14 days of room temperature storage. Top: Representative analysis, for Donor 2, of gene expression profile of more than 47000 transcripts, using the Ilumina Human HT12 Bead Array, for RNA extracted from blood collected in RNAgard Blood or competitor’s Tubes after 3 days (A,C) or 14 days (B,D) of room temperature storage, relative to RNA extracted from freshly collected blood, using RNA isolation methods recommended for each product. Bottom: Table summarizing the microarray results. Displayed are the number of genes with > 2-fold change in expression levels relative to expression levels on the day of blood sample collection.

RNA yield from BioMaxi Blood RNA Purification Kit

Figure 2. RNA yield analyzed by UV spectroscopy. RNA amounts are for whole tube (2.5 mL blood) isolating using BioMaxi Blood RNA Purification kit after 14 days of storage at ambient temperature.

14-day RNA protection at ambient temperatures

 

Figure 3. Multiple donor comparison of RNAgard Blood Tubes (RGB) versus Competitor P (P) after 14 days of ambient temperature storage. 200ng of RNA is visualized on a 1.2% agarose gel. BioMaxi Blood RNA Purification kit was used for isolating RNA from RNAgard Blood Tubes.

RNAgard Blood System: Reproducible and Repeatable RNA Purification: RNA Purity (A260/A280) for 3 Operators (A, B, C)

Figure 4. RNA purity obtained using the RNAgard Blood System is highly reproducible. Blood was collected from 8 healthy donors. After 3, 7 or 15 days of sample storage, RNA was purified from duplicate samples. RNA purity of each individual sample was determined by UV spectrophotometry (A260/A280). On Day 3, duplicate samples for each donor were purified by 3 different operators showing reproducibility of our system.

Stability of specific RNA transcripts isolated with RNAgard Blood System

Figure 5. RNAgard Blood Tube maintains unaltered gene expression for 14 days at 18-25°C. Blood from two healthy donors was collected in RNAgard tubes and purified with BioMaxi RNA after the indicated storage time. Gene expression of C-Fos (A) and IL1-β (B), normalized to 18S rRNA, was determined by qPCR at the indicated storage time, relative to day 0 samples.

RNA Stability after 7 weeks at 4°C

Figure 6. RNA yield of whole blood from 5 different donors was tested using the RNAgard Blood System following storage for 7 weeks at 4°C versus control (Day = 0). Data shows that the quality and yield of RNA following 7 weeks of storage in RNAgard Blood Tubes at 4°C were equivalent to that of RNA purified from whole blood on the day of blood draw.

Impact of freeze / thaw conditions on the integrity of RNAgard Blood Tubes

Table 1. RNAgard Blood Tubes remain intact after freezer storage for 7 days. Blood was collected from two healthy donors into RNAgard Blood Tubes. The tubes were then kept at room temperature for at least 2 hours before freezing down. Four different freezing methods were tested, and the tubes were stored frozen as indicated for 7 days. The tubes were thawed using three different methods. No tube damages (cracks, splits, etc.) were observed.

Stable gene expression >47,000 transcripts

Tested on Illumina Human HT-12 v4 Array

Tested on Illumina Human HT-12 v4 Array

Gene Transcript: BCR-ABL
Functional Test: RT-qPCR

Functional testing with a gene expression-based cancer diagnostic test.

RNA yield (70% higher with RNAgard)

RNA Quality

RNA Integrity

Specifications

RNAgard Blood Tubes, CE

  • Evacuated, sterile, blood collection tubes (10mL)
  • Pre-fill volume (preservative) = 6.65mL
  • Blood draw volume = 2.5mL
  • Pre-fill storage temperature = 18°C – 25°C
  • RNA preservation at room temperature = 14 days
  • For Export Only. Not for sale in the U.S.

RNAgard Blood Tubes

  • Evacuated, sterile, blood collection tubes (10mL)
  • Pre-fill volume (preservative) = 6.65mL
  • Blood draw volume = 2.5mL
  • Pre-fill storage temperature = 18°C – 25°C*
  • RNA preservation at room temperature = 14 days
  • For Research Use Only. Not for use in diagnostic procedures.

*For post-fill storage at temperatures below -20°C, use cryovials.

Resources

RNAgard Blood System

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AN - RNAgard® Blood Application Note 439.67 KB

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Brochure - RNAgard® Product Overview 1.04 MB

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HP - RNAgard® Blood Purification Kit User Manual 1.00 MB

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HP - RNAgard® Blood Reagent IFU 265.58 KB

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HP - RNAgard® Blood RNA Precipitation Buffer IFU 244.69 KB

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HP - RNAgard® Blood Tubes IFU 143.84 KB

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SDS - RNAgard® Blood RNA Purification Kit SDS 597.55 KB

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SDS - RNAgard® Blood System MSDS 121.09 KB

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SDS - RNAgard® Blood Tube and Reagent SDS 120.74 KB

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SDS - RNAgard® RNA Purification Kit Precipitation Buffer SDS 121.09 KB

    RNAgard Blood System CE

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    Brochure - RNAgard® Product Overview 1.04 MB

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    HP - RNAgard® Blood Purification Kit, CE User Manual 999.11 KB

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    HP - RNAgard® Blood Purification Kit, CE User Manual (French) 1.10 MB

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    HP - RNAgard® Blood Purification Kit, CE User Manual (German) 1.11 MB

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    HP - RNAgard® Blood Purification Kit, CE User Manual (Italian) 1.05 MB

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    HP - RNAgard® Blood Purification Kit, CE User Manual (Spanish) 1.05 MB

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    HP - RNAgard® Blood Reagent, CE IFU 207.52 KB

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    HP – RNAgard® Blood Reagent, CE IFU (French) 268.80 KB

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    HP – RNAgard® Blood Reagent, CE IFU (German) 194.06 KB

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    HP – RNAgard® Blood Reagent, CE IFU (Italian) 271.04 KB

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    HP – RNAgard® Blood Reagent, CE IFU (Spanish) 212.01 KB

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    HP - RNAgard® Blood RNA Precipitation Buffer CE IFU 517.31 KB

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    HP – RNAgard® Blood RNA Precipitation Buffer CE IFU (French) 244.40 KB

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    HP – RNAgard® Blood RNA Precipitation Buffer CE IFU (German) 297.86 KB

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    HP – RNAgard® Blood RNA Precipitation Buffer CE IFU (Italian) 239.23 KB

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    HP – RNAgard® Blood RNA Precipitation Buffer CE IFU (Spanish) 184.72 KB

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    HP - RNAgard® Blood Tubes, CE IFU 158.17 KB

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    HP - RNAgard® Blood Tubes, CE IFU (French) 226.88 KB

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    HP - RNAgard® Blood Tubes, CE IFU (German) 299.63 KB

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    HP - RNAgard® Blood Tubes, CE IFU (Italian) 288.04 KB

    Application Notes

    Ordering

    Non-CE Version

    For Research Use Only. Not for use in diagnostic procedures in the U.S.

    • Catalog No.: 12006-051
    • Description: RNAgard Blood Tubes & Precipitation Buffer
    • Size: 50-pack + 180mL
    • Price: $150.00
    • Order Here

     

    • Catalog No.: 64201-601
    • Description: RNAgard Blood Purification Kit
    • Size:  50 preps
    • Price: $445.00
    • Order Here

     

    • Catalog No.: 66201-151
    • Description: RNAgard Blood Reagent
    • Size: 100mL
    • Price: $150.00
    • Order Here

    CE Version – For Export Only. Not for sale in the U.S

    • Catalog No.: CE-12006-051
    • Description: RNAgard Blood Tubes & Precipitation Buffer, CE
    • Size: 50-pack + 180mL Buffer
    • Price: $395.00
    • Order Here
    • Catalog No.: CE-12000-001
    • Description: RNAgard Blood Purification Kit, CE
    • Size: 50 preps
    • Price:  $445.00
    • Order Here
    • Catalog No.: CE-1210-001
    • Description: RNAgard Blood Reagent, CE
    • Size: 100mL
    • Price: $180.00
    • Order Here

    BIOMATRICA
    5627 Oberlin Drive
    Suite 120
    San Diego, CA 92121
    Tel: 858-550-0308
    Fax: 858-678-0597
    Email: orders@biomatrica.com