RNAstable® / LD

Stabilize and store RNA at Ambient Temperatures

  • Preserves RNA at ambient temperatures without degradation
  • Eliminates the need to store RNA in freezers or to ship on dry ice
  • Concentrates RNA more effectively than other methods
  • Compatible with most downstream applications
Category:

Overview

RNAstable® and RNAstable® LD allows you to preserve and ship your total RNA, mRNA, and miRNA for up to 12 years at ambient temperatures*. They provide a cost-effective, eco-friendly solution to freezer crawl and lab space management. Moreover, they elimnate sample loss due to freeze/thaw cycles. When you are ready to use your RNA samples, you can fully recover them in minutes without any additional purification steps.

RNAstable is available in two convenient formats:

  • RNAstable: provided pre-dried in 1.5 ml microfuge tubes and 96-well plates to which hydrated RNA can be added.
  • RNAstable LD: a liquid reagent which can be added directly to RNA samples in your own tubes, multi-well plates, or other containers

Product Data

Figure 1: RNAstable protects total RNA, mRNA, miRNA. Samples can be safely stored for 12 years at ambient temperature and protected from degradation.

Figure 2: Successful gene expression analysis on microarray.

Figure 3: (A) Aliquots of total RNA were stored for 29months at either -80°C or at room temperature protected in RNAstable or unprotected. RNA integrity was assessed using agarose gel electrophoresis and bioanalyzer analysis (e.g. RIN score). (B) Aliquots of the stored samples were quantified using the 18S rRNA gene in a One-Step RT-PCR reaction. Three samples were amplified for each storage condition and the results from the 9 reactions are overlaid on the graph. (C) Recovery levels (pg) for total RNA stored under identical conditions.

Specifications

  • Product formats:
    • Pre-dried in 1.5 ml microfuge tubes and 96-well plates (RNAstable)
    • Liquid aliquots of 2 ml and 10 ml (RNAstable LD)
  • Application volume: 100μl
  • Application amount: 0.01 to 100µg
  • Drying time: Overnight (laminar flow hood); 0.5 to 1.5 hours with vacuum concentrator system (speedVac®)
  • Recovery volume: 10 to 100μl
  • Recovery: ∼99.9%
  • DNA storage at room temperature: 12 years accelerated aging; 3.5 years “real time”

Resources

RNAstable

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AN - Improved QPCR Analysis Following Storage in RNAstable® 99.13 KB

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AN - Microarray Analysis of RNA in RNAstable® 105.65 KB

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AN - RNA Stabilization at Room Temperature in RNAstable® 244.70 KB

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AN - Summary of RNAstable validation studies 869.56 KB

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BioTechniques - August 2009 - Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis 769.71 KB

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BioTechniques - June 2010 - Stabilizing RNA at room temperature in RNAstable® 651.25 KB

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Brochure - RNAstable® / RNAstable® LD Overview 873.52 KB

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HP - RNAstable® Quick Reference Protocol 1.08 MB

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HP - RNAstable®/RNAstable® LD Handbook 109.15 KB

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microRNA (miRNA) Stabilization in RNAstable® 295.80 KB

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miRNA Sequencing with RNAstable® and SeqMatic miRNA kit 372.49 KB

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RNAstable® Frequently Asked Questions (FAQ's) 51.42 KB

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SDS - RNAstable® SDS 140.82 KB

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Stabilization of poly(A) mRNA transcripts in RNAstable® 150.21 KB

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Summary of research evaluations of RNA preservation in RNAstable® versus cold storage (at -80°C) 869.56 KB

    RNAstable LD

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    Brochure - RNAstable® / RNAstable® LD Overview 873.52 KB

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    HP - RNAstable® LD Quick Reference Protocol 119.09 KB

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    HP - RNAstable®/RNAstable® LD Handbook 109.15 KB

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    RNAstable® Frequently Asked Questions (FAQ's) 51.42 KB

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    SDS - RNAstable® LD SDS 140.82 KB

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    Stabilization of large volume RNA samples dried in 96-well deep well plates with RNAstable® LD 384.76 KB

      Ordering

      • Catalog No.: 93221-001
      • Description: RNAstable® Tube Kit
      • Size: (25) Tubes of RNAstable®
      • Price: $100.00
      • Order Here
      • Catalog No.: 93221-011
      • Description: RNAstable® Tube Kit
      • Size: 3 x (25) Tubes of RNAstable®
      • Price: $225.00
      • Order Here
      • Catalog No.: 90220-001
      • Description: RNAstable® 96-Well Plate
      • Size: (1) 96-well plates (Min. Qty: 2)
      • Price: $201.00
      • Order Here
      • Catalog No.: 16901-081
      • Description: Breathe-EASIER Membranes
      • Size: 100-pack
      • Price: Please Inquire
      • Catalog No.: 53201-013
      • Description: RNAstable® LD
      • Size: 2 mL
      • Price: $410.00
      • Order Here
      • Catalog No.: 52201-013
      • Description: RNAstable® LD
      • Size: 10 mL
      • Price: $1,230.00
      • Order Here

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      Tel: 858-550-0308
      Fax: 858-678-0597
      Email: orders@biomatrica.com