RNA stable™ and RNA stable™ LD allows you to preserve and ship your total RNA, mRNA, and miRNA for up to 12 years at ambient temperatures*. They provide a cost-effective, eco-friendly solution to freezer crawl and lab space management. Moreover, they eliminate sample loss due to freeze/thaw cycles. When you are ready to use your RNA samples, you can fully recover them in minutes without any additional purification steps.
RNA stable is available in two convenient formats:
RNA stable: provided pre-dried in 1.5 ml microfuge tubes and 96-well plates to which hydrated RNA can be added.
RNA stable LD: a liquid reagent which can be added directly to RNA samples in your own tubes, multi-well plates, or other containers
Figure 1: RNA stable protects total RNA, mRNA, miRNA. Samples can be safely stored for 12 years at ambient temperature and protected from degradation.
Figure 2: Successful gene expression analysis on microarray.
Figure 3: (A) Aliquots of total RNA were stored for 29 months at either -80°C or at room temperature protected in RNA stable or unprotected. RNA integrity was assessed using agarose gel electrophoresis and bioanalyzer analysis (e.g. RIN score). (B) Aliquots of the stored samples were quantified using the 18S rRNA gene in a One-Step RT-PCR reaction. Three samples were amplified for each storage condition and the results from the 9 reactions are overlaid on the graph. (C) Recovery levels (pg) for total RNA stored under identical conditions.
Pre-dried in 1.5 ml microfuge tubes and 96-well plates (RNA stable)
Liquid aliquots of 2 ml and 10 ml (RNA stable LD)
Application volume: 100μl
Application amount: 0.01 to 100µg
Drying time: Overnight (laminar flow hood); 0.5 to 1.5 hours with vacuum concentrator system (speedVac®)
Recovery volume: 10 to 100μl
DNA storage at room temperature: 12 years accelerated aging; 3.5 years “real time”
Touch the yellow matrix with an RNAase-free pipette tip. If yellow does not stick to the tip, then RNA stable is completely dry. If yellow sticks to the tip, then RNA stable is not completely dry. Allow sample to continue drying.